Quantitative determination of total phenolic content in. Development and validation of a hplc method for the. Phytochemical and hptlc studies of methanolic extract of. New sources of polyphenols with anticancer, antioxidant, and antimicrobial properties in arid environments are critical for the development of alternative medicines and natural remedies. In vitro antioxidant activity of mentha pulegium from saudi. Antibacterial activity of ethyl acetate and aqueous extracts of mentha longifolia l. Chemical composition and biological activities of mentha. Development of hplc analytical protocol for artemisinin quantification in plant materials and extracts summary alexei a. The highest percentage extract was yielded from mentha longifolia 18. This study explored the polyphenol profiles and biological activities of methanolic leaf extracts from natural mentha.
Methanolic extract indicated significant activity against staphylococcus aureus, klebsiella pneumonia and candida albicans. High performance liquid chromatography waters alliance 2695 and mass spectrometry. Hplc analysis is the most popularly uses method for analysis of compounds as it is high sensitivity and selectivity. Comparative study of mentha species growing wild in egypt. Phytochemical and hptlc studies of methanolic extract of indigofera tinctoria fabaceae f. Aggarwal4 the present study was undertaken to investigate the diuretic effect of methanolic extract of the dried leaves of salvadora persica in normal rats. Department of biology, faculty of sciences, shahid chamran university, ahvaz, iran. The distribution of phenolic compounds in the methanolic extract showed a variation among studied plants. Isolation and identification of antimicrobial compound. Antioxidant and cytotoxic potential of methanol extract of.
Phytochemical are the dependable sources for the treatment of different health problem. The phytochemical constituents and antibacterial effects of the methanolic leaf extract of citrus aurantifolia were evaluated in the present study. The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. The largest inhibition zones of mentha longifolia were observed for ethanol extract against staphylococcus aureus 7mm and corynibacteria 4mm. The study was carried out to evaluate the phytochemical constituents and antioxidant potential of methanolic root extract of mentha arvensis l.
Analysis was carried out using reverse phase hplc at 254 nm and a gradient mobile phase. Antimycotic activities of selected plant flora, growing. Investigation of antibacterial activity of ethanolic and. To develop a reverse phase high performance liquid chromatography hplc method for the determination of eurycomanone in e. The initial concentration was obtained by dissolving 0. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay. Phytochemical investigation of essential oils from ethanolic extract of polyalthia longifolia by gas. The aqueous methanolic extracts of the two egyptian species were subjected to a comparative study for the first time using the lcesims fingerprint.
Page 159 hplc analysis of methanolic extract of herbs for quercetin content neelam verma1, nitu trehan2 1. The content of total phenolics mg tannic acid equivalent per g dry weight of the sample differed from 50. In this study, the variability of morphological parameters, essential oil composition, and phenolic compounds such as rosmarinic acid and caffeic acid content were assessed. The aim of this study was to investigate the effect of aerial parts of stachys lavandulifolia vahl. The chemical constituents identified by the gcms analysis of the methanolic plant extract of mentha spicata l. Phytochemical investigation of essential oils from. Antioxidant activities and rphplc identification of polyphenols in the methanolic extract of mentha genus article pdf available august 2015 with 456 reads how we measure reads. Gcms analysis of phytocomponents in the methanolic extract of. Polyphenol profile and antimicrobial and cytotoxic.
Hplc uv method development and quantification of eugenol from methanolic extracts of some spices farhin inam, sujata deo, neha. The efficacy of pe extracts against all pathogens tested was higher than that of methanolic extracts. Specificity specificity is the ability of a method to discriminate between the study analytes and other components in the sample. Additionally, hplc identification was implemented for effective compounds in all aqueous extracts. Quantitative determination of total phenolic content in stem bark and leaves extracts of madhuca longifolia sharma p, chaturvedi n2, upadhyay m3, varma s4 department of food science and nutrition, banasthali university, district, tonk, rajasthan304022, india corres. The powder was macerated using absolute methanol hplc grade, fisher scientific, uk. The maximum of phenol production was reached during the full flowering stage.
Simultaneous quantitative determination of cinnamaldehyde. Isolation and purification of alkaloids from medicinal plants by hplc vinod borde, babasaheb sonwane, vrushali sontakke and bharthi somwanshi department of biotechnology, vinayakrao patil college, vaijapur, dist. Gcms analysis of phytocomponents in the methanolic extract of moringa oleifera leave. The essential oil showed strong antimicrobial activity against all 30 microorganisms tested whereas the methanol extract almost remained inactive. Preparation of methanolic extract prior to extraction process, the airdried samples were pulver.
Standard antibiotics ciprofloxacin 10mcgdisc and ketoconazole 10mcgdisc showed good inhibitory action on the microorganisms tested. The extracts were prepared with three solvents of different polarity n hexane. Isolation and purification of alkaloids from medicinal plants. Phytochemical analysis of methanolic extracts of leaves of. Phytochemical analysis revealed the presence of alkaloids, saponins, phenols, flavonoids, terpenoids. Validated rphplc method for quantification of phenolic. Biotechnology department, punjabi university, patiala, india. Antihypertensive and toxicity studies of aqueous methanolic extract of mentha longifolia l article pdf available in journal of animal and plant sciences 236. Polyphenol profile and antimicrobial and cytotoxic activities. Petroleum ether pe and methanolic extracts of nine wild plant species were tested in vitro for their antimycotic activity against eight phytopathogenic fungi.
Pdf cytotoxic activity of methanolic extract of mentha. The present research work was conducted to appraise the antithrombotic activity of mentha longifolia using in vitro and in vivo experiments. Antimicrobial and antioxidant properties of the essential oils and methanol extract from mentha longifolia l. Some parts of this plant have been used in traditional medicine of iran and other countries. Lamiaceae is distributed all over the world and can be found in many environments. The antioxidant activity of methanol extract of mentha l. Gcms analysis of methanolic extract was done by standard protocol using the equipment perkinelmer gas chromatographymass spectrometry, while the mass spectra of the compounds found in the extract was matched with the national institute of standards and technology nist library. The antioxidative potential of the samples was evaluated using dpph and abts assays. Nevertheless, at fructification stage, the highest antioxidant. Development and validation of a hplc method for the quantifi. Pdf antioxidant activities and rphplc identification of.
Camellia sinensis methanolic and aqueous extracts noor s. This study investigated total phenolic and antioxidant activity of five iranian mint accessions from two different species, mentha spicata l. Prophylactic and therapeutic uses of egyptian mentha. Isolation and purification of alkaloids from medicinal. Hplc analysis of flavonoids in eupatorium littorale. Mentha oil and menthol are important ingredients in a wide range of cosmetic and pharmaceutical preparations chadha and gupta, 1995, mainly for their refreshing properties.
Gcms analysis of bioactive compounds in methanolic. This study evaluated the antioxidant and antibacterial activity of green tea by using the methanolic and aqueous extract. Isolation and purification of alkaloids from medicinal plants by hplc vinod borde, babasaheb sonwane, vrushali sontakke and bharthi somwanshi. Mansour seyyednejad,1 fariba dehghani,2 zahra hasannejad2 1. Many studies have shown various pharmacological and therapeutic effects of the plant. Mentha longifolia herba definition mentha longifolia herba consists of the dried aerial parts of mentha longifolia l.
The hydroalcoholic extract of this plant had no antibacterial activity against 2. Antioxidant activities and rp hplc identification of polyphenols in the methanolic extract of mentha genus article pdf available august 2015 with 456 reads how we measure reads. Cytotoxic activity of methanolic extract of mentha longifolia and ocimum basilicum against human breast cancer article pdf available in pakistan journal of biological sciences 1623. The bioactive compounds of solanum torvum leaves have been evaluated using gc ms and ft ir. The results of the present investigation showed differences between five mentha species cultivated in romania that could be important for their therapeutical valorification, like antioxidant or.
Methanolic extracts of 28 medicinal plants were evaluated for the presence of. Determination of phytocomponents in methanolic extract of. Phytochemical analysis of methanolic extracts of leaves of some medicinal plants anubha arora department of botany v. Quantitative determination of total phenolic content in stem.
Antibacterial and antioxidant properties of the methanolic. The essential oil is used as flavouring component in a wide range of household and oral products. Antimicrobial activity and biochemical profiling of selected. The twenty accessions in this study have been coded from g1 to g20, and the geographical attributes of their locations, recorded by gps device are compiled in table 1. Phenological stage effect on phenolic composition and. The methanolic extract of this plant at 24 mg effective dose had more effect on b. The method was validated according to ich harmonised tripartite guidelines validation of analytical procedures. The crude extract was prepared by maceration method 90% methanol followed by fractionating into chloroform, ethyl acetate, petroleum ether and water. Comparison of total phenolic and antioxidant activity of. Development of hplc analytical protocol for artemisinin. A simple, sensitive, and precise reversedphase high performance liquid chromatographic hplc method has been developed, validated and used for simultaneous quantitative determination of cinnamaldehyde and methyl eugenol from the methanolic extract of dried bark powder of cinnamomum zeylanicum blume family lauraceae. Phytochemical screening and gcms, ftir analysis of.
In vitro antioxidant activity of mentha pulegium from. Quantitative determination of total phenolic content in stem bark and leaves extracts of madhuca longifolia. Latteef institute of genetic engineering and biotechnology for postgraduate studies baghdad university, baghdad, iraq. In the present study, an antimicrobial compound was isolated and characterized, and its biological activity was. Hplc uv method development and quantification of eugenol from methanolic extracts of some spices farhin inam, sujata deo, neha narkhede 98 preparation of working standard solution of eugenol intermediate solution of 100 gml was prepared from stock solution of gml. This study was designed to evaluate antimicrobial and antioxidant activities of the essential oil and methanol extract from mentha longifolia ssp. Pdf antithrombotic activity of mentha longifolia in. Phytochemical analysis of methanolic extracts of leaves of some medicinal plants sudipa nag, anirban paul and rituparna dutta department of botany, rampurhat college, rampurhat, birbhum, west bengal, india. On the other hand, the ethanolic extract of mentha longifolia and olea europaea showed higher activities than methanolic extract. Antioxidant activity and volatile and phenolic profiles of.
Apigenin was the major phenol in mentha pulegium with an amount of 6. The main phytoactive compounds of methanolic extract were caryophyllene, tcadinol, menthone, eugenol and menthol crotonate. Reverse phase high performance liquid chromatography for the. Mentha longifolia wild mint is a popular folk remedy. Lcmsbased metabolite profiling of methanolic extracts. Phytochemical investigation of essential oils from ethanolic extract of polyalthia longifolia by gas chromatographymass spectroscopy. Abstract the present investigation deals with the phytochemical studies of leaves of different medicinal plants like andrographis. The highest effect of methanol extract against streptococcus viridian 3. External standard curves for authentic samples of eupafolin and.
The fresh samples were airdried at 25 c in a dark place and then stored at 18 c. A hplc method for determination of ursolic acid and betulinic. Mentha species, one of the worlds oldest and most popular herbs, are widely used in cooking, in cosmetics, and as alternative or complementary therapy, mainly for the treatment of gastrointestinal disorders like flatulence, indigestion, nausea, vomiting, anorexia, and ulcerative colitis. Blue colour was observed for gallic tannins and green black for catecholic tannins iyengar, 1995. Total phenolic and flavonoid content of mentha pulegium methanol extract were also determined. The antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown mentha longifolia m. Hplc uv method development and quantification of eugenol. Lcmsbased metabolite profiling of methanolic extracts from.
The samples were collected from the sudan, khartoum province. Wild marjoram origanum syriacum pe extract showed the highest and widest range of activity. Phytochemical analysis of the leaves of tabernaemontana. Cytotoxic activity of methanolic extract of mentha longifolia and ocimum. Pdf antihypertensive and toxicity studies of aqueous. Antimicrobial activity and biochemical profiling of. The extract obtained from the aerial parts reduced dpph with a halfmaximal inhibition concentration ic 50 of 0. Anticandidal and anticarcinogenic activities of mentha. Muthulingam department of zoology, faculty of science, annamalai university, annamalainagar, tn india abstract the aerial parts fresh leaves of the plant indigofera tinctoria were collected powdered and the powder was initially. The same reaction mixture without the methanol extract but the equivalent amount of methanol served as the control. Pdf comparative study of mentha species growing wild in.
Chandra, in handbook of herbs and spices second edition, volume 1, 2012. Lamiaceae leaves have been traditionally implemented in the treatment of minor sore throat and minor mouth or throat irritation by the indigenous people of iraq, although the compounds responsible for the medicinal properties have not been identified. Barau department of science laboratory technology, school of technology, kano state polytechnic abstract the whole part of the plant caralluma longidens family of asclepiadaceae was collected from samaru, zaria. Methanol was used as the solvent for extract preparation, which was further, implemented for antibacterial and phytochemical assays. Peaks were observed in the methanolic extract in the similar retention time corresponding to the values as. The total phenolic concentration of the extracts were determined using folin ciocalteu method. Reverse phase high performance liquid chromatography for. Antimicrobial and antioxidant properties of the essential. Investigation of antibacterial activity of ethanolic and methanolic extracts of mentha pulegium l. The antioxidant activity of the methanolic extract and fractions was assessed by the dpph and carotenelinoleic acid assays.
Barau department of science laboratory technology, school of technology, kano state polytechnic abstract the whole part of the plant caralluma longidens. The dried powder of citrus aurantifolia leaves was used for extract preparation. Phytochemical investigation of essential oils from ethanolic. Examples of highperformance liquid chromatography with diodearray detection hplc. Pharmacological and therapeutic effects of mentha longifolia. Material and methods the selected plant species were carefully identified with the help of available floras hooker 18721897. Effect of solvent types and extraction times on phenolic. Phytochemical, antibacterial and antioxidant activity of. Preliminary phytochemical and antibacterial screening of.
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